Media
Movie 1
GFP:tubulin time-lapse sequence starting before nuclear envelope breakdown and showing spindle assembly and early translocation to the cortex. Spindle shortening and rotation during meiosis I and II are shown but these are not as clear as in
Movie 2
GFP:tubulin time-lapse sequence starting after nuclear envelope breakdown. After the sequence stops jumping around, the parallel orientation of the metaphase spindle, shortening and rotation can be seen clearly through meiosis I and II.
Movie 3
DIC time-lapse sequence showing nuclear envelope breakdown, ovulation, and circular streaming of membrane vesicles during meiosis. The appearance of pronuclei at the end of the movie indicates exit from meiosis.
Video S1
Video s2 KCA-1
Female meiosis and centrosome maturation in kca-1::aid::gfp meiotic embryo treated with auxin.
In utero time-lapse movie with GFP:: SPCS-1 signal peptidase (green) and mKate::Tubulin (magenta) labeled.
Combined stacks_8-31-2023_10_complete
Meiosis I filmed in utero with the combination of TOMM-20::GFP::SSPB and K420::mKate::iLID in the unc-116(g-null) background. TOMM-20::GFP::SSPB has been pseudo-colored in cyan and K420::mKate::iLID is in red. Tubulin and PH are also in red. Grayscale of both constructs are seen tangential to the merge video. Mitochondria are seen to be packed into a tight ball at the future posterior end of the oocyte. During ovulation the mitochondria remain packed toward the center and the spindle is localized at the cortex during metaphase I and anaphase I. Bar = 10 μm
KCA-1 localization n VIT_ 7-19-2023_1_KCA_Tub_Yolk_Combined
Meiosis I filmed in utero in a strain endogenously tagged with KCA-1::AID::GFP. Yolk granules are endogenously tagged with VIT-2::Halo. Tubulin and Ph are labeled in red. Before ovulation KCA-1::AID::GFP in grayscale can be seen on vesicles that co-localize with yolk granules. During ovulation KCA-1::AID::GFP comes off the vesicles and appears to remain on filaments in the uterus. In the uterus yolk granules are packed in towards the center of the meiotic embryo. Bar = 10 μm
TOMM-20-SSPB unc-116(gk5722) FM___ GFP200 RFP170 2x2 bin 1-24-2024_11_label.avi
Meiosis I filmed in utero with TOMM-20::GFP::SSPB in unc-116(g-null) background pseudo colored in cyan and tubulin in red. The meiotic spindle is centered during metaphase I and reaches the cortex during anaphase I. Mitochondria are relaxed out toward the cortex. Bar = 10 μm
Movie 4
GFP:vitellogenin time-lapse sequence showing meiosis-specific circular streaming of lysosomes. In this sequence, lysosomes are streaming in the short axis of the embryo.
Movie 5
GFP:vitellogenin time-lapse sequence showing meiosis-specific circular streaming of lysosomes. In this sequence, lysosomes are streaming in the long axis of the embryo.
Movie 6
GFP:tubulin time-lapse sequence showing movement of cytoplasmic microtubules during streaming in a meiotic embryo……
Movie 7
GFP:tubulin and mCherry:histone time lapse sequence showing a meiotic spindle from pro-metaphase I, through spindle translocation, shortening, rotation, and most of anaphase I.
Movie 8
GFP:tubulin, GFP:PH (plasma membrane), and mCherry:histone time lapse sequence showing the events of anaphase I in an embryo with two unpaired X chromosomes. A single chromosome appears to be lagging through anaphase.
Movie 9
GFP:tubulin, GFP:PH (plasma membrane), and mCherry:histone time lapse sequence showing the events of anaphase I in an embryo with two unpaired X chromosomes. A single chromosome lags and is resolved into the forming polar body.